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KMID : 1134820110400081086
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 8 p.1086 ~ p.1091
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Inhibitory Effects of Ethanol Extracts from Polygoni multiflori radix and Cynanchi wilfordii radix on Melanogenesis in Melanoma Cells
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Seo Hee
Seo Geun-Young
Ko Su-Zie
Park Young-Hyun
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Abstract
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Anti-oxidative activity and tyrosinase inhibitory activity of various ethanol extracts of Polygoni multiflori radix (PMR) and Cynanchi wilfordii radix (CWR) were compared to identify an anti-oxidant and whitening agent source from nature. We conducted an investigation into the anti-oxidant activities of PMR and CWR ethanol extracts by measuring total polyphenol content, total flavonoid content, and ABTS radical capacity. The total polyphenol contents of PMR and CWR were 17.31±0.54 ㎎ GA/eq g, and 2.75±0.22 ㎎ GA/eq g, respectively. The total flavonoid contents of PMR and CWR were 6.38±0.39 ㎎ naringine/eq g, and 1.34±0.09 ㎎ naringine/eq g, respectively. The 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical decolorization of PMR and CWR were 96.89±0.21% at 1 ㎎/㎖ and 93.49±0.76% at 50 ㎎/㎖. Melanoma cells were cultured with the PMR and CWR ethanol extracts for 48 hr, and total melanin content as a final product and the activity of tyrosinase, a key enzyme, in melanogenesis, were estimated. The PMR and CWR ethanol extracts increased melanin content and tyrosinase activity in a dose-dependent manner. These results suggest that PMR and CWR ethanol extracts could be useful as a skin whitening agent.
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KEYWORD
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Polygoni multiflori radix, Cynanchi wilfordii radix, melanin, tyrosinase, antioxidant
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